Detailed information of TA system TA111027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2408259..2408964
• Replicon: chromosome
• Accession: NZ_CP032078
• Organism: Escherichia coli strain CT30

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: D2195_RS11555
• Protein ID: WP_000539521.1
• Coordinates: 2408578..2408964 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D2195_RS11550
• Protein ID: WP_001280945.1
• Coordinates: 2408259..2408588 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2195_RS11535 (2403416)	2403416..2404327	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
D2195_RS11540 (2404505)	2404505..2406853	+	2349	Protein_2279	EAL domain-containing protein	-
D2195_RS11545 (2406861)	2406861..2408189	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
D2195_RS11550 (2408259)	2408259..2408588	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
D2195_RS11555 (2408578)	2408578..2408964	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D2195_RS11560 (2409190)	2409190..2410515	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
D2195_RS11565 (2410728)	2410728..2411111	+	384	WP_077520521.1	biofilm formation regulator BssR	-
D2195_RS11570 (2411222)	2411222..2412337	+	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
D2195_RS11575 (2412334)	2412334..2412960	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T111027 WP_000539521.1 NZ_CP032078:c2408964-2408578 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T111027 NZ_CP032078:c2408964-2408578 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT111027 WP_001280945.1 NZ_CP032078:c2408588-2408259 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT111027 NZ_CP032078:c2408588-2408259 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6