Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1999982..2000600 Replicon chromosome
Accession NZ_CP032078
Organism Escherichia coli strain CT30

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D2195_RS09500 Protein ID WP_001291435.1
Coordinates 1999982..2000200 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A8S7S0Y3
Locus tag D2195_RS09505 Protein ID WP_039004128.1
Coordinates 2000226..2000600 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2195_RS09465 (1995271) 1995271..1995843 + 573 WP_000779821.1 YbaY family lipoprotein -
D2195_RS09470 (1995874) 1995874..1996185 - 312 WP_000409911.1 MGMT family protein -
D2195_RS09480 (1996564) 1996564..1996917 + 354 WP_000878140.1 DUF1428 domain-containing protein -
D2195_RS09485 (1996959) 1996959..1998509 - 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D2195_RS09490 (1998673) 1998673..1999143 - 471 WP_000136192.1 YlaC family protein -
D2195_RS09495 (1999259) 1999259..1999810 - 552 WP_000102564.1 maltose O-acetyltransferase -
D2195_RS09500 (1999982) 1999982..2000200 - 219 WP_001291435.1 HHA domain-containing protein Toxin
D2195_RS09505 (2000226) 2000226..2000600 - 375 WP_039004128.1 Hha toxicity modulator TomB Antitoxin
D2195_RS09510 (2001146) 2001146..2004295 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
D2195_RS09515 (2004318) 2004318..2005511 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111025 WP_001291435.1 NZ_CP032078:c2000200-1999982 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T111025 NZ_CP032078:c2000200-1999982 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14587.42 Da        Isoelectric Point: 4.7395

>AT111025 WP_039004128.1 NZ_CP032078:c2000600-2000226 [Escherichia coli]
MDEYSPKRHDITQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT111025 NZ_CP032078:c2000600-2000226 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCACGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure

References