Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1448304..1448929 Replicon chromosome
Accession NZ_CP032078
Organism Escherichia coli strain CT30

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag D2195_RS06940 Protein ID WP_000911329.1
Coordinates 1448531..1448929 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag D2195_RS06935 Protein ID WP_000450524.1
Coordinates 1448304..1448531 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2195_RS06910 (1444104) 1444104..1444574 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
D2195_RS06915 (1444574) 1444574..1445146 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
D2195_RS06920 (1445292) 1445292..1446170 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
D2195_RS06925 (1446187) 1446187..1447221 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
D2195_RS06930 (1447434) 1447434..1448147 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
D2195_RS06935 (1448304) 1448304..1448531 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
D2195_RS06940 (1448531) 1448531..1448929 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
D2195_RS06945 (1449076) 1449076..1449939 + 864 WP_001267498.1 neutral zinc metallopeptidase -
D2195_RS06950 (1449954) 1449954..1451969 + 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
D2195_RS06955 (1452043) 1452043..1452741 + 699 WP_000679819.1 esterase -
D2195_RS06960 (1452822) 1452822..1453022 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T111024 WP_000911329.1 NZ_CP032078:1448531-1448929 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T111024 NZ_CP032078:1448531-1448929 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT111024 WP_000450524.1 NZ_CP032078:1448304-1448531 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT111024 NZ_CP032078:1448304-1448531 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References