Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 997263..997917 Replicon chromosome
Accession NZ_CP032078
Organism Escherichia coli strain CT30

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag D2195_RS04915 Protein ID WP_000244781.1
Coordinates 997510..997917 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D2195_RS04910 Protein ID WP_000354046.1
Coordinates 997263..997529 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2195_RS04890 (993341) 993341..994774 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
D2195_RS04895 (994819) 994819..995130 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
D2195_RS04900 (995294) 995294..995953 + 660 WP_000250269.1 hemolysin III family protein -
D2195_RS04905 (996030) 996030..997010 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
D2195_RS04910 (997263) 997263..997529 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D2195_RS04915 (997510) 997510..997917 + 408 WP_000244781.1 protein YgfX Toxin
D2195_RS04920 (997957) 997957..998478 - 522 WP_001055874.1 flavodoxin FldB -
D2195_RS04925 (998590) 998590..999486 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D2195_RS04930 (999511) 999511..1000221 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D2195_RS04935 (1000227) 1000227..1001960 + 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T111023 WP_000244781.1 NZ_CP032078:997510-997917 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T111023 NZ_CP032078:997510-997917 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT111023 WP_000354046.1 NZ_CP032078:997263-997529 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT111023 NZ_CP032078:997263-997529 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References