Detailed information of TA system TA111013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 8311..8909
• Replicon: plasmid p.CT29-P1
• Accession: NZ_CP032074
• Organism: Escherichia coli strain CT29

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: D2194_RS22420
• Protein ID: WP_053270478.1
• Coordinates: 8311..8688 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: D2194_RS22425
• Protein ID: WP_001603498.1
• Coordinates: 8688..8909 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2194_RS22395 (D2194_24195)	3336..3689	+	354	WP_187180131.1	hypothetical protein	-
D2194_RS22400 (D2194_24200)	3728..4405	-	678	WP_187180117.1	hypothetical protein	-
D2194_RS22405 (D2194_24205)	4779..5504	+	726	WP_187180116.1	hypothetical protein	-
D2194_RS22410 (D2194_24210)	5565..6905	+	1341	WP_097296309.1	DnaB-like helicase C-terminal domain-containing protein	-
D2194_RS22415 (D2194_24215)	6993..8177	+	1185	WP_023135654.1	hypothetical protein	-
D2194_RS22420 (D2194_24225)	8311..8688	-	378	WP_053270478.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
D2194_RS22425 (D2194_24230)	8688..8909	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D2194_RS22430 (D2194_24235)	9094..10104	-	1011	WP_089615833.1	fimbrial protein	-
D2194_RS22435 (D2194_24240)	10115..12601	-	2487	WP_212403473.1	fimbrial biogenesis outer membrane usher protein	-
D2194_RS22440 (D2194_24245)	12619..13299	-	681	WP_032187672.1	fimbrial assembly chaperone	-
D2194_RS22445 (D2194_24250)	13355..13903	-	549	WP_212403475.1	fimbrial protein YehD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108175	108175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13419.20 Da
• Isoelectric Point: 6.7260

>T111013 WP_053270478.1 NZ_CP032074:c8688-8311 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITGLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T111013 NZ_CP032074:c8688-8311 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGTC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGGCCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT111013 WP_001603498.1 NZ_CP032074:c8909-8688 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT111013 NZ_CP032074:c8909-8688 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGTCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0