Detailed information of TA system TA111012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 1547..2345
• Replicon: plasmid p.CT29-P1
• Accession: NZ_CP032074
• Organism: Escherichia coli strain CT29

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: D2194_RS22380
• Protein ID: WP_032187665.1
• Coordinates: 1547..2068 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: D2194_RS22385
• Protein ID: WP_001711191.1
• Coordinates: 2076..2345 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2194_RS22365 (D2194_24160)	95..418	+	324	WP_001711185.1	hypothetical protein	-
D2194_RS22370 (D2194_24165)	432..1124	+	693	WP_024172707.1	membrane protein	-
D2194_RS22375 (D2194_24170)	1127..1378	+	252	WP_023135660.1	hypothetical protein	-
D2194_RS22380 (D2194_24180)	1547..2068	-	522	WP_032187665.1	GNAT family N-acetyltransferase	Toxin
D2194_RS22385 (D2194_24185)	2076..2345	-	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
D2194_RS22390 (D2194_24190)	2665..3330	+	666	WP_023135658.1	AAA family ATPase	-
D2194_RS22395 (D2194_24195)	3336..3689	+	354	WP_187180131.1	hypothetical protein	-
D2194_RS22400 (D2194_24200)	3728..4405	-	678	WP_187180117.1	hypothetical protein	-
D2194_RS22405 (D2194_24205)	4779..5504	+	726	WP_187180116.1	hypothetical protein	-
D2194_RS22410 (D2194_24210)	5565..6905	+	1341	WP_097296309.1	DnaB-like helicase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108175	108175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19345.11 Da
• Isoelectric Point: 7.7881

>T111012 WP_032187665.1 NZ_CP032074:c2068-1547 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T111012 NZ_CP032074:c2068-1547 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAACGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCTAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT111012 WP_001711191.1 NZ_CP032074:c2345-2076 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT111012 NZ_CP032074:c2345-2076 [Escherichia coli]
ATGTCCGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2