Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3430484..3431102 Replicon chromosome
Accession NZ_CP032073
Organism Escherichia coli strain CT29

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D2194_RS16565 Protein ID WP_001291435.1
Coordinates 3430884..3431102 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag D2194_RS16560 Protein ID WP_000344800.1
Coordinates 3430484..3430858 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2194_RS16550 (3425573) 3425573..3426766 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D2194_RS16555 (3426789) 3426789..3429938 + 3150 WP_196056373.1 efflux RND transporter permease AcrB -
D2194_RS16560 (3430484) 3430484..3430858 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
D2194_RS16565 (3430884) 3430884..3431102 + 219 WP_001291435.1 HHA domain-containing protein Toxin
D2194_RS16570 (3431274) 3431274..3431825 + 552 WP_000102564.1 maltose O-acetyltransferase -
D2194_RS16575 (3431941) 3431941..3432411 + 471 WP_000136192.1 YlaC family protein -
D2194_RS16580 (3432575) 3432575..3434125 + 1551 WP_077477703.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D2194_RS16585 (3434167) 3434167..3434520 - 354 Protein_3280 DUF1428 family protein -
D2194_RS16595 (3434899) 3434899..3435210 + 312 WP_000409911.1 MGMT family protein -
D2194_RS16600 (3435241) 3435241..3435813 - 573 WP_077477690.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111008 WP_001291435.1 NZ_CP032073:3430884-3431102 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T111008 NZ_CP032073:3430884-3431102 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT111008 WP_000344800.1 NZ_CP032073:3430484-3430858 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT111008 NZ_CP032073:3430484-3430858 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References