Detailed information of TA system TA111006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3144631..3145269
• Replicon: chromosome
• Accession: NZ_CP032073
• Organism: Escherichia coli strain CT29

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: D2194_RS15185
• Protein ID: WP_000813794.1
• Coordinates: 3144631..3144807 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D2194_RS15190
• Protein ID: WP_001270286.1
• Coordinates: 3144853..3145269 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2194_RS15165 (3140250)	3140250..3141464	-	1215	WP_071597387.1	BenE family transporter YdcO	-
D2194_RS15170 (3141517)	3141517..3142053	+	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
D2194_RS15175 (3142126)	3142126..3144087	+	1962	WP_212403047.1	U32 family peptidase	-
D2194_RS15180 (3144179)	3144179..3144409	-	231	WP_000494244.1	YncJ family protein	-
D2194_RS15185 (3144631)	3144631..3144807	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D2194_RS15190 (3144853)	3144853..3145269	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D2194_RS15195 (3145348)	3145348..3146754	+	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
D2194_RS15200 (3146999)	3146999..3148144	+	1146	WP_087902524.1	ABC transporter substrate-binding protein	-
D2194_RS15205 (3148162)	3148162..3149175	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
D2194_RS15210 (3149176)	3149176..3150117	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3138945..3140210	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T111006 WP_000813794.1 NZ_CP032073:3144631-3144807 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T111006 NZ_CP032073:3144631-3144807 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT111006 WP_001270286.1 NZ_CP032073:3144853-3145269 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT111006 NZ_CP032073:3144853-3145269 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB