Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 966386..967221 Replicon chromosome
Accession NZ_CP032073
Organism Escherichia coli strain CT29

Toxin (Protein)


Gene name yeeV Uniprot ID -
Locus tag D2194_RS04650 Protein ID WP_057109034.1
Coordinates 966844..967221 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1E5MC43
Locus tag D2194_RS04645 Protein ID WP_063112944.1
Coordinates 966386..966754 (+) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2194_RS04605 (961579) 961579..962463 + 885 WP_032216515.1 50S ribosome-binding GTPase -
D2194_RS04610 (962582) 962582..963259 + 678 WP_063091580.1 hypothetical protein -
D2194_RS04615 (963265) 963265..963417 + 153 WP_001696589.1 DUF905 family protein -
D2194_RS04620 (963518) 963518..964336 + 819 WP_001234688.1 DUF932 domain-containing protein -
D2194_RS04625 (964418) 964418..964897 + 480 WP_000860074.1 antirestriction protein -
D2194_RS04630 (964913) 964913..965389 + 477 WP_001366830.1 RadC family protein -
D2194_RS04635 (965452) 965452..965673 + 222 WP_000692323.1 DUF987 domain-containing protein -
D2194_RS04640 (965692) 965692..966336 + 645 WP_045146861.1 hypothetical protein -
D2194_RS04645 (966386) 966386..966754 + 369 WP_063112944.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
D2194_RS04650 (966844) 966844..967221 + 378 WP_057109034.1 TA system toxin CbtA family protein Toxin
D2194_RS04655 (967218) 967218..967367 + 150 Protein_925 DUF5983 family protein -
D2194_RS04660 (967443) 967443..967640 + 198 WP_023563519.1 DUF957 domain-containing protein -
D2194_RS04665 (967725) 967725..968567 + 843 WP_001529559.1 DUF4942 domain-containing protein -
D2194_RS04670 (968849) 968849..969385 - 537 WP_000942785.1 GspM family type II secretion system protein YghD -
D2194_RS04675 (969387) 969387..970565 - 1179 Protein_929 type II secretion system protein GspL -
D2194_RS04680 (970562) 970562..971539 - 978 WP_001411127.1 type II secretion system minor pseudopilin GspK -
D2194_RS04685 (971542) 971542..972141 - 600 WP_001255034.1 type II secretion system minor pseudopilin GspJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(19-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 13934.86 Da        Isoelectric Point: 6.8517

>T110996 WP_057109034.1 NZ_CP032073:966844-967221 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Download         Length: 378 bp

>T110996 NZ_CP032073:966844-967221 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13724.52 Da        Isoelectric Point: 6.8270

>AT110996 WP_063112944.1 NZ_CP032073:966386-966754 [Escherichia coli]
VSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT110996 NZ_CP032073:966386-966754 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCACGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A1E5MC43

References