Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 845809..846463 Replicon chromosome
Accession NZ_CP032073
Organism Escherichia coli strain CT29

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag D2194_RS04070 Protein ID WP_000244781.1
Coordinates 845809..846216 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D2194_RS04075 Protein ID WP_000354046.1
Coordinates 846197..846463 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2194_RS04050 (841766) 841766..843499 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
D2194_RS04055 (843505) 843505..844215 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D2194_RS04060 (844240) 844240..845136 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D2194_RS04065 (845248) 845248..845769 + 522 WP_001055874.1 flavodoxin FldB -
D2194_RS04070 (845809) 845809..846216 - 408 WP_000244781.1 protein YgfX Toxin
D2194_RS04075 (846197) 846197..846463 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D2194_RS04080 (846706) 846706..847686 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
D2194_RS04085 (847882) 847882..848541 - 660 WP_000250274.1 hemolysin III family protein -
D2194_RS04090 (848705) 848705..849016 - 312 WP_212403265.1 N(4)-acetylcytidine aminohydrolase -
D2194_RS04095 (849061) 849061..850494 + 1434 WP_032278899.1 6-phospho-beta-glucosidase BglA -
D2194_RS04100 (850551) 850551..851294 - 744 WP_186185804.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T110995 WP_000244781.1 NZ_CP032073:c846216-845809 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T110995 NZ_CP032073:c846216-845809 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCTTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT110995 WP_000354046.1 NZ_CP032073:c846463-846197 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT110995 NZ_CP032073:c846463-846197 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References