Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 55778..56271 | Replicon | plasmid p.E166-P3 |
| Accession | NZ_CP032069 | ||
| Organism | Escherichia coli strain E166 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9Y9W8 |
| Locus tag | D2J89_RS25455 | Protein ID | WP_001230709.1 |
| Coordinates | 56017..56271 (-) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | U9Y4R8 |
| Locus tag | D2J89_RS25450 | Protein ID | WP_000151456.1 |
| Coordinates | 55778..56020 (-) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D2J89_RS25415 (D2J89_27540) | 51101..51394 | - | 294 | WP_000954637.1 | hypothetical protein | - |
| D2J89_RS25420 (D2J89_27545) | 51409..51783 | - | 375 | WP_015387341.1 | hypothetical protein | - |
| D2J89_RS25425 (D2J89_27550) | 51835..52122 | - | 288 | WP_000673430.1 | hypothetical protein | - |
| D2J89_RS25430 (D2J89_27555) | 52254..52472 | - | 219 | WP_154101533.1 | hypothetical protein | - |
| D2J89_RS25435 (D2J89_27560) | 52872..54134 | + | 1263 | WP_000608644.1 | IS1380-like element ISEcp1 family transposase | - |
| D2J89_RS25440 (D2J89_27565) | 54387..55262 | + | 876 | WP_032492042.1 | extended-spectrum class A beta-lactamase CTX-M-64 | - |
| D2J89_RS25445 (D2J89_27570) | 55309..55653 | - | 345 | Protein_65 | WbuC family cupin fold metalloprotein | - |
| D2J89_RS25450 (D2J89_27575) | 55778..56020 | - | 243 | WP_000151456.1 | hypothetical protein | Antitoxin |
| D2J89_RS25455 (D2J89_27580) | 56017..56271 | - | 255 | WP_001230709.1 | hypothetical protein | Toxin |
| D2J89_RS25460 (D2J89_27585) | 56268..56810 | - | 543 | WP_001083904.1 | hypothetical protein | - |
| D2J89_RS25465 (D2J89_27590) | 56914..57429 | - | 516 | WP_001025393.1 | J domain-containing protein | - |
| D2J89_RS25470 (D2J89_27600) | 57833..58162 | - | 330 | WP_000855119.1 | hypothetical protein | - |
| D2J89_RS25475 (D2J89_27605) | 58294..58512 | - | 219 | WP_159183630.1 | hypothetical protein | - |
| D2J89_RS25480 (D2J89_27610) | 58749..59414 | + | 666 | WP_015387337.1 | division plane positioning ATPase MipZ | - |
| D2J89_RS25485 | 59488..59772 | + | 285 | WP_001162371.1 | hypothetical protein | - |
| D2J89_RS25490 (D2J89_27620) | 59790..60386 | - | 597 | WP_000125165.1 | ProQ/FINO family protein | - |
| D2J89_RS25495 (D2J89_27625) | 60535..60657 | - | 123 | WP_223202818.1 | Hok/Gef family protein | - |
| D2J89_RS26015 | 60602..60754 | - | 153 | Protein_76 | DUF5431 family protein | - |
| D2J89_RS25500 | 60890..61051 | - | 162 | WP_001128311.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | mcr-1.1 / blaCTX-M-64 | - | 1..63623 | 63623 | |
| - | flank | IS/Tn | blaCTX-M-64 | - | 52872..55262 | 2390 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 9942.47 Da Isoelectric Point: 10.1206
>T110991 WP_001230709.1 NZ_CP032069:c56271-56017 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
Download Length: 255 bp
>T110991 NZ_CP032069:c56271-56017 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA
Antitoxin
Download Length: 81 a.a. Molecular weight: 9136.45 Da Isoelectric Point: 7.2775
>AT110991 WP_000151456.1 NZ_CP032069:c56020-55778 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
Download Length: 243 bp
>AT110991 NZ_CP032069:c56020-55778 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9H8F0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9GNF5 |