Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 20139..20664 | Replicon | plasmid p.E166-P2 |
| Accession | NZ_CP032068 | ||
| Organism | Escherichia coli strain E166 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | V0SSI5 |
| Locus tag | D2J89_RS24315 | Protein ID | WP_001159868.1 |
| Coordinates | 20139..20444 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | S1PPD8 |
| Locus tag | D2J89_RS24320 | Protein ID | WP_000813634.1 |
| Coordinates | 20446..20664 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D2J89_RS24270 (15371) | 15371..15694 | + | 324 | Protein_13 | helix-turn-helix domain-containing protein | - |
| D2J89_RS24275 (15696) | 15696..15800 | + | 105 | Protein_14 | AAA family ATPase | - |
| D2J89_RS24280 (15861) | 15861..16374 | + | 514 | Protein_15 | DUF4113 domain-containing protein | - |
| D2J89_RS24285 (16376) | 16376..16792 | - | 417 | WP_001278813.1 | plasmid partitioning/stability family protein | - |
| D2J89_RS24290 (16785) | 16785..17147 | - | 363 | Protein_17 | plasmid segregation protein ParM | - |
| D2J89_RS24295 (17203) | 17203..17900 | + | 698 | WP_106425952.1 | IS1-like element IS1A family transposase | - |
| D2J89_RS24300 (18113) | 18113..18421 | - | 309 | WP_000030198.1 | hypothetical protein | - |
| D2J89_RS24305 (18508) | 18508..19152 | - | 645 | WP_001144036.1 | ParA family protein | - |
| D2J89_RS24310 (19332) | 19332..20138 | - | 807 | WP_001593069.1 | site-specific integrase | - |
| D2J89_RS24315 (20139) | 20139..20444 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| D2J89_RS24320 (20446) | 20446..20664 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| D2J89_RS24325 (21492) | 21492..21782 | - | 291 | WP_001229374.1 | hypothetical protein | - |
| D2J89_RS24330 (21782) | 21782..22912 | - | 1131 | WP_000542418.1 | DUF4238 domain-containing protein | - |
| D2J89_RS24335 (22942) | 22942..24045 | - | 1104 | WP_001127573.1 | nucleotide-binding protein | - |
| D2J89_RS24340 (24373) | 24373..24630 | + | 258 | WP_000371889.1 | hypothetical protein | - |
| D2J89_RS24345 (24630) | 24630..25220 | + | 591 | WP_000194553.1 | hypothetical protein | - |
| D2J89_RS24350 (25240) | 25240..25587 | - | 348 | WP_000142449.1 | DUF6404 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sitABCD / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 | iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB | 1..163224 | 163224 | |
| - | flank | IS/Tn | - | - | 15371..15727 | 356 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11706.51 Da Isoelectric Point: 6.4674
>T110982 WP_001159868.1 NZ_CP032068:c20444-20139 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T110982 NZ_CP032068:c20444-20139 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT110982 WP_000813634.1 NZ_CP032068:c20664-20446 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT110982 NZ_CP032068:c20664-20446 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|