Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20139..20664 Replicon plasmid p.E166-P2
Accession NZ_CP032068
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag D2J89_RS24315 Protein ID WP_001159868.1
Coordinates 20139..20444 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag D2J89_RS24320 Protein ID WP_000813634.1
Coordinates 20446..20664 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS24270 (15371) 15371..15694 + 324 Protein_13 helix-turn-helix domain-containing protein -
D2J89_RS24275 (15696) 15696..15800 + 105 Protein_14 AAA family ATPase -
D2J89_RS24280 (15861) 15861..16374 + 514 Protein_15 DUF4113 domain-containing protein -
D2J89_RS24285 (16376) 16376..16792 - 417 WP_001278813.1 plasmid partitioning/stability family protein -
D2J89_RS24290 (16785) 16785..17147 - 363 Protein_17 plasmid segregation protein ParM -
D2J89_RS24295 (17203) 17203..17900 + 698 WP_106425952.1 IS1-like element IS1A family transposase -
D2J89_RS24300 (18113) 18113..18421 - 309 WP_000030198.1 hypothetical protein -
D2J89_RS24305 (18508) 18508..19152 - 645 WP_001144036.1 ParA family protein -
D2J89_RS24310 (19332) 19332..20138 - 807 WP_001593069.1 site-specific integrase -
D2J89_RS24315 (20139) 20139..20444 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
D2J89_RS24320 (20446) 20446..20664 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
D2J89_RS24325 (21492) 21492..21782 - 291 WP_001229374.1 hypothetical protein -
D2J89_RS24330 (21782) 21782..22912 - 1131 WP_000542418.1 DUF4238 domain-containing protein -
D2J89_RS24335 (22942) 22942..24045 - 1104 WP_001127573.1 nucleotide-binding protein -
D2J89_RS24340 (24373) 24373..24630 + 258 WP_000371889.1 hypothetical protein -
D2J89_RS24345 (24630) 24630..25220 + 591 WP_000194553.1 hypothetical protein -
D2J89_RS24350 (25240) 25240..25587 - 348 WP_000142449.1 DUF6404 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB 1..163224 163224
- flank IS/Tn - - 15371..15727 356


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T110982 WP_001159868.1 NZ_CP032068:c20444-20139 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T110982 NZ_CP032068:c20444-20139 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT110982 WP_000813634.1 NZ_CP032068:c20664-20446 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT110982 NZ_CP032068:c20664-20446 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References