Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 110994..111637 Replicon plasmid p.E166-P1
Accession NZ_CP032067
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag D2J89_RS23955 Protein ID WP_001044768.1
Coordinates 111221..111637 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag D2J89_RS23950 Protein ID WP_001261287.1
Coordinates 110994..111224 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS23930 (D2J89_25820) 106154..107242 + 1089 WP_000952217.1 transcriptional repressor PifC -
D2J89_RS23935 (D2J89_25825) 107244..109469 + 2226 WP_000698737.1 P-loop NTPase fold protein -
D2J89_RS23940 (D2J89_25830) 109519..110418 - 900 WP_000963206.1 nucleotide-binding protein -
D2J89_RS23945 (D2J89_25835) 110408..110698 - 291 WP_000111771.1 hypothetical protein -
D2J89_RS23950 (D2J89_25845) 110994..111224 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
D2J89_RS23955 (D2J89_25850) 111221..111637 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
D2J89_RS23960 (D2J89_25855) 111799..113937 - 2139 WP_000350638.1 AAA family ATPase -
D2J89_RS23965 (D2J89_25860) 114291..114548 + 258 WP_000343085.1 hypothetical protein -
D2J89_RS23970 (D2J89_25865) 114548..115138 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / fosA3 / oqxA / oqxB / aph(3')-IIa - 1..157787 157787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T110981 WP_001044768.1 NZ_CP032067:111221-111637 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T110981 NZ_CP032067:111221-111637 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT110981 WP_001261287.1 NZ_CP032067:110994-111224 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT110981 NZ_CP032067:110994-111224 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References