Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 88902..89424 Replicon plasmid p.E166-P1
Accession NZ_CP032067
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag D2J89_RS23830 Protein ID WP_000220561.1
Coordinates 88902..89183 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag D2J89_RS23835 Protein ID WP_000121743.1
Coordinates 89173..89424 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS23800 (D2J89_25650) 84767..85399 + 633 WP_000535208.1 hypothetical protein -
D2J89_RS23805 (D2J89_25655) 85446..86444 - 999 WP_155061724.1 hypothetical protein -
D2J89_RS23810 (D2J89_25660) 86444..86890 - 447 Protein_83 DnaB-like helicase C-terminal domain-containing protein -
D2J89_RS23815 (D2J89_25665) 87008..87196 + 189 WP_000957857.1 hypothetical protein -
D2J89_RS23820 (D2J89_25670) 87206..88405 + 1200 WP_000948429.1 IS91 family transposase -
D2J89_RS23825 88571..88663 + 93 Protein_86 IS6 family transposase -
D2J89_RS23830 (D2J89_25680) 88902..89183 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D2J89_RS23835 (D2J89_25685) 89173..89424 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
D2J89_RS23840 (D2J89_25695) 90659..91498 + 840 WP_024129959.1 replication initiation protein -
D2J89_RS23845 (D2J89_25700) 91519..92361 + 843 WP_000435064.1 replication initiation protein -
D2J89_RS23850 (D2J89_25705) 92365..92811 + 447 WP_001074384.1 hypothetical protein -
D2J89_RS23855 (D2J89_25710) 92948..93301 + 354 WP_024129958.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / fosA3 / oqxA / oqxB / aph(3')-IIa - 1..157787 157787
- flank IS/Tn - - 87206..88405 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T110980 WP_000220561.1 NZ_CP032067:c89183-88902 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T110980 NZ_CP032067:c89183-88902 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT110980 WP_000121743.1 NZ_CP032067:c89424-89173 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT110980 NZ_CP032067:c89424-89173 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References