Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | relBE/ParE-DnaT |
Location | 88902..89424 | Replicon | plasmid p.E166-P1 |
Accession | NZ_CP032067 | ||
Organism | Escherichia coli strain E166 |
Toxin (Protein)
Gene name | relE | Uniprot ID | D0QMR1 |
Locus tag | D2J89_RS23830 | Protein ID | WP_000220561.1 |
Coordinates | 88902..89183 (-) | Length | 94 a.a. |
Antitoxin (Protein)
Gene name | relB | Uniprot ID | G3CAG1 |
Locus tag | D2J89_RS23835 | Protein ID | WP_000121743.1 |
Coordinates | 89173..89424 (-) | Length | 84 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
D2J89_RS23800 (D2J89_25650) | 84767..85399 | + | 633 | WP_000535208.1 | hypothetical protein | - |
D2J89_RS23805 (D2J89_25655) | 85446..86444 | - | 999 | WP_155061724.1 | hypothetical protein | - |
D2J89_RS23810 (D2J89_25660) | 86444..86890 | - | 447 | Protein_83 | DnaB-like helicase C-terminal domain-containing protein | - |
D2J89_RS23815 (D2J89_25665) | 87008..87196 | + | 189 | WP_000957857.1 | hypothetical protein | - |
D2J89_RS23820 (D2J89_25670) | 87206..88405 | + | 1200 | WP_000948429.1 | IS91 family transposase | - |
D2J89_RS23825 | 88571..88663 | + | 93 | Protein_86 | IS6 family transposase | - |
D2J89_RS23830 (D2J89_25680) | 88902..89183 | - | 282 | WP_000220561.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
D2J89_RS23835 (D2J89_25685) | 89173..89424 | - | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
D2J89_RS23840 (D2J89_25695) | 90659..91498 | + | 840 | WP_024129959.1 | replication initiation protein | - |
D2J89_RS23845 (D2J89_25700) | 91519..92361 | + | 843 | WP_000435064.1 | replication initiation protein | - |
D2J89_RS23850 (D2J89_25705) | 92365..92811 | + | 447 | WP_001074384.1 | hypothetical protein | - |
D2J89_RS23855 (D2J89_25710) | 92948..93301 | + | 354 | WP_024129958.1 | DNA distortion polypeptide 3 | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Mobilizable plasmid | mph(A) / fosA3 / oqxA / oqxB / aph(3')-IIa | - | 1..157787 | 157787 | |
- | flank | IS/Tn | - | - | 87206..88405 | 1199 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11023.86 Da Isoelectric Point: 10.5938
>T110980 WP_000220561.1 NZ_CP032067:c89183-88902 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR
Download Length: 282 bp
>T110980 NZ_CP032067:c89183-88902 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT110980 WP_000121743.1 NZ_CP032067:c89424-89173 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT110980 NZ_CP032067:c89424-89173 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A3S5I302 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A3S5I301 |