Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 67765..68366 Replicon plasmid p.E166-P1
Accession NZ_CP032067
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag D2J89_RS23710 Protein ID WP_001216045.1
Coordinates 67765..68145 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag D2J89_RS23715 Protein ID WP_001190712.1
Coordinates 68145..68366 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS25945 62863..62982 - 120 WP_071527722.1 ash family protein -
D2J89_RS23685 (D2J89_25525) 63205..64689 - 1485 WP_000124155.1 hypothetical protein -
D2J89_RS23690 (D2J89_25530) 64689..65882 - 1194 WP_016236876.1 terminase A protein -
D2J89_RS23695 (D2J89_25535) 65969..66421 - 453 WP_016236875.1 hypothetical protein -
D2J89_RS23700 (D2J89_25540) 66510..67553 - 1044 WP_000648821.1 DUF968 domain-containing protein -
D2J89_RS23705 (D2J89_25545) 67581..67760 - 180 WP_000113018.1 hypothetical protein -
D2J89_RS23710 (D2J89_25550) 67765..68145 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
D2J89_RS23715 (D2J89_25555) 68145..68366 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D2J89_RS23720 (D2J89_25560) 68549..70105 + 1557 WP_016236874.1 type I restriction-modification system subunit M -
D2J89_RS23725 (D2J89_25565) 70102..71307 + 1206 WP_001293319.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / fosA3 / oqxA / oqxB / aph(3')-IIa - 1..157787 157787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T110979 WP_001216045.1 NZ_CP032067:c68145-67765 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T110979 NZ_CP032067:c68145-67765 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT110979 WP_001190712.1 NZ_CP032067:c68366-68145 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT110979 NZ_CP032067:c68366-68145 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGTCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References