Detailed information of TA system TA110977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4761984..4762620
• Replicon: chromosome
• Accession: NZ_CP032066
• Organism: Escherichia coli strain E166

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: D2J89_RS22840
• Protein ID: WP_000813796.1
• Coordinates: 4761984..4762160 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D2J89_RS22845
• Protein ID: WP_076838470.1
• Coordinates: 4762204..4762620 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2J89_RS22820 (4757606)	4757606..4758778	-	1173	WP_001236216.1	BenE family transporter YdcO	-
D2J89_RS22825 (4758870)	4758870..4759406	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
D2J89_RS22830 (4759479)	4759479..4761440	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D2J89_RS22835 (4761532)	4761532..4761762	-	231	WP_000494243.1	YncJ family protein	-
D2J89_RS22840 (4761984)	4761984..4762160	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D2J89_RS22845 (4762204)	4762204..4762620	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D2J89_RS22850 (4762699)	4762699..4764105	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
D2J89_RS22855 (4764350)	4764350..4765495	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
D2J89_RS22860 (4765513)	4765513..4766526	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
D2J89_RS22865 (4766527)	4766527..4767468	+	942	WP_086525100.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T110977 WP_000813796.1 NZ_CP032066:4761984-4762160 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T110977 NZ_CP032066:4761984-4762160 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT110977 WP_076838470.1 NZ_CP032066:4762204-4762620 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT110977 NZ_CP032066:4762204-4762620 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3