Detailed information of TA system TA110976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4442246..4443030
• Replicon: chromosome
• Accession: NZ_CP032066
• Organism: Escherichia coli strain E166

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: D2J89_RS21255
• Protein ID: WP_000613624.1
• Coordinates: 4442246..4442740 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: D2J89_RS21260
• Protein ID: WP_001110446.1
• Coordinates: 4442737..4443030 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2J89_RS21235 (4437439)	4437439..4438536	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
D2J89_RS21240 (4438536)	4438536..4439477	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
D2J89_RS21245 (4439543)	4439543..4441186	+	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
D2J89_RS21250 (4441198)	4441198..4442151	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
D2J89_RS21255 (4442246)	4442246..4442740	-	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
D2J89_RS21260 (4442737)	4442737..4443030	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
D2J89_RS21265 (4443164)	4443164..4446349	-	3186	WP_000827395.1	ribonuclease E	-
D2J89_RS21270 (4446922)	4446922..4447881	+	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T110976 WP_000613624.1 NZ_CP032066:c4442740-4442246 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T110976 NZ_CP032066:c4442740-4442246 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGATCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT110976 WP_001110446.1 NZ_CP032066:c4443030-4442737 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT110976 NZ_CP032066:c4443030-4442737 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTATCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775