Detailed information of TA system TA110975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4188937..4189642
• Replicon: chromosome
• Accession: NZ_CP032066
• Organism: Escherichia coli strain E166

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: D2J89_RS20040
• Protein ID: WP_000539521.1
• Coordinates: 4189256..4189642 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D2J89_RS20035
• Protein ID: WP_001280945.1
• Coordinates: 4188937..4189266 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2J89_RS20020 (4184094)	4184094..4185005	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
D2J89_RS20025 (4185183)	4185183..4187531	+	2349	Protein_3936	EAL domain-containing protein	-
D2J89_RS20030 (4187539)	4187539..4188867	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
D2J89_RS20035 (4188937)	4188937..4189266	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
D2J89_RS20040 (4189256)	4189256..4189642	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D2J89_RS20045 (4189868)	4189868..4191193	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
D2J89_RS20050 (4191406)	4191406..4191789	+	384	WP_000497137.1	biofilm formation regulator BssR	-
D2J89_RS20055 (4191900)	4191900..4193015	+	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
D2J89_RS20060 (4193012)	4193012..4193638	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T110975 WP_000539521.1 NZ_CP032066:c4189642-4189256 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T110975 NZ_CP032066:c4189642-4189256 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT110975 WP_001280945.1 NZ_CP032066:c4189266-4188937 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT110975 NZ_CP032066:c4189266-4188937 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6