Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3111999..3112624 Replicon chromosome
Accession NZ_CP032066
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag D2J89_RS14830 Protein ID WP_000911329.1
Coordinates 3112226..3112624 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag D2J89_RS14825 Protein ID WP_000450524.1
Coordinates 3111999..3112226 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS14800 (3107797) 3107797..3108267 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
D2J89_RS14805 (3108267) 3108267..3108839 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
D2J89_RS14810 (3108985) 3108985..3109863 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
D2J89_RS14815 (3109880) 3109880..3110914 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
D2J89_RS14820 (3111127) 3111127..3111840 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
D2J89_RS14825 (3111999) 3111999..3112226 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
D2J89_RS14830 (3112226) 3112226..3112624 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
D2J89_RS14835 (3112771) 3112771..3113634 + 864 WP_001267498.1 neutral zinc metallopeptidase -
D2J89_RS14840 (3113649) 3113649..3115664 + 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
D2J89_RS14845 (3115738) 3115738..3116436 + 699 WP_000679819.1 esterase -
D2J89_RS14850 (3116517) 3116517..3116717 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T110965 WP_000911329.1 NZ_CP032066:3112226-3112624 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T110965 NZ_CP032066:3112226-3112624 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT110965 WP_000450524.1 NZ_CP032066:3111999-3112226 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT110965 NZ_CP032066:3111999-3112226 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References