Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2669764..2670418 Replicon chromosome
Accession NZ_CP032066
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag D2J89_RS12855 Protein ID WP_000244781.1
Coordinates 2670011..2670418 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D2J89_RS12850 Protein ID WP_000354046.1
Coordinates 2669764..2670030 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS12830 (2665842) 2665842..2667275 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
D2J89_RS12835 (2667320) 2667320..2667631 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
D2J89_RS12840 (2667795) 2667795..2668454 + 660 WP_000250269.1 hemolysin III family protein -
D2J89_RS12845 (2668531) 2668531..2669511 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
D2J89_RS12850 (2669764) 2669764..2670030 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D2J89_RS12855 (2670011) 2670011..2670418 + 408 WP_000244781.1 protein YgfX Toxin
D2J89_RS12860 (2670458) 2670458..2670979 - 522 WP_001055874.1 flavodoxin FldB -
D2J89_RS12865 (2671091) 2671091..2671987 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D2J89_RS12870 (2672012) 2672012..2672722 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D2J89_RS12875 (2672728) 2672728..2674461 + 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T110964 WP_000244781.1 NZ_CP032066:2670011-2670418 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T110964 NZ_CP032066:2670011-2670418 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT110964 WP_000354046.1 NZ_CP032066:2669764-2670030 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT110964 NZ_CP032066:2669764-2670030 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References