Detailed information of TA system TA110960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2010781..2011412
• Replicon: chromosome
• Accession: NZ_CP032066
• Organism: Escherichia coli strain E166

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: D2J89_RS09545
• Protein ID: WP_001260301.1
• Coordinates: 2010781..2011056 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: D2J89_RS09550
• Protein ID: WP_000593557.1
• Coordinates: 2011053..2011412 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2J89_RS09530 (2005785)	2005785..2006852	+	1068	WP_000361470.1	HlyD family secretion protein	-
D2J89_RS09535 (2006849)	2006849..2009584	+	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
D2J89_RS09540 (2009584)	2009584..2010708	+	1125	WP_001318088.1	ABC transporter permease	-
D2J89_RS09545 (2010781)	2010781..2011056	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
D2J89_RS09550 (2011053)	2011053..2011412	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D2J89_RS09555 (2011532)	2011532..2011933	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
D2J89_RS09560 (2011939)	2011939..2012745	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
D2J89_RS09565 (2012742)	2012742..2013506	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
D2J89_RS09570 (2013506)	2013506..2014339	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
D2J89_RS09575 (2014336)	2014336..2015280	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T110960 WP_001260301.1 NZ_CP032066:2010781-2011056 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T110960 NZ_CP032066:2010781-2011056 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT110960 WP_000593557.1 NZ_CP032066:2011053-2011412 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT110960 NZ_CP032066:2011053-2011412 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1