Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1211720..1212217 Replicon chromosome
Accession NZ_CP032066
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag D2J89_RS05900 Protein ID WP_001173343.1
Coordinates 1211720..1211893 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag D2J89_RS05905 Protein ID WP_001317561.1
Coordinates 1211921..1212217 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS05890 (1208390) 1208390..1209847 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
D2J89_RS05895 (1210084) 1210084..1211601 + 1518 WP_000003805.1 lysine--tRNA ligase -
D2J89_RS05900 (1211720) 1211720..1211893 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
D2J89_RS05905 (1211921) 1211921..1212217 - 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
D2J89_RS05910 (1212444) 1212444..1212716 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
D2J89_RS05915 (1212728) 1212728..1212958 - 231 WP_000371717.1 4Fe-4S mono-cluster protein YjdI -
D2J89_RS05920 (1213138) 1213138..1214769 + 1632 WP_001216477.1 sensor histidine kinase -
D2J89_RS05925 (1214766) 1214766..1215485 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1202866..1212217 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T110955 WP_001173343.1 NZ_CP032066:c1211893-1211720 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T110955 NZ_CP032066:c1211893-1211720 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT110955 WP_001317561.1 NZ_CP032066:c1212217-1211921 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT110955 NZ_CP032066:c1212217-1211921 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References