Detailed information of TA system TA110954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX(toxin)
• Location: 997327..998141
• Replicon: chromosome
• Accession: NZ_CP032066
• Organism: Escherichia coli strain E166

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: D2J89_RS04850
• Protein ID: WP_001054376.1
• Coordinates: 997327..997584 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: A0A8E0IVE5
• Locus tag: D2J89_RS04855
• Protein ID: WP_001350781.1
• Coordinates: 997596..998141 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2J89_RS04830 (992615)	992615..993721	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
D2J89_RS04835 (993786)	993786..994766	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
D2J89_RS25770 (994876)	994876..995081	+	206	Protein_951	HNH endonuclease	-
D2J89_RS04840 (995349)	995349..996589	-	1241	Protein_952	helicase YjhR	-
D2J89_RS25775 (996705)	996705..996836	+	132	WP_001309182.1	hypothetical protein	-
D2J89_RS04850 (997327)	997327..997584	+	258	WP_001054376.1	YjhX family toxin	Toxin
D2J89_RS04855 (997596)	997596..998141	+	546	WP_001350781.1	N-acetyltransferase	Antitoxin
D2J89_RS04860 (998197)	998197..998943	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
D2J89_RS04865 (999112)	999112..999330	+	219	Protein_957	hypothetical protein	-
D2J89_RS04870 (999368)	999368..999484	+	117	Protein_958	VOC family protein	-
D2J89_RS04875 (999729)	999729..1000850	+	1122	WP_000010832.1	M42 family metallopeptidase	-
D2J89_RS04880 (1000847)	1000847..1001125	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
D2J89_RS04885 (1001137)	1001137..1002450	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	987112..1006365	19253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T110954 WP_001054376.1 NZ_CP032066:997327-997584 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T110954 NZ_CP032066:997327-997584 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19974.91 Da
• Isoelectric Point: 6.3277

>AT110954 WP_001350781.1 NZ_CP032066:997596-998141 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAFIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT110954 NZ_CP032066:997596-998141 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCGGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCTTTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82