Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 656050..656848 Replicon chromosome
Accession NZ_CP032066
Organism Escherichia coli strain E166

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag D2J89_RS03250 Protein ID WP_000854730.1
Coordinates 656471..656848 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag D2J89_RS03245 Protein ID WP_001285481.1
Coordinates 656050..656424 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2J89_RS03205 (652025) 652025..652477 + 453 WP_000682723.1 hypothetical protein -
D2J89_RS03210 (652595) 652595..652828 + 234 WP_001213776.1 DUF905 family protein -
D2J89_RS03215 (652928) 652928..653749 + 822 WP_001234359.1 DUF932 domain-containing protein -
D2J89_RS03220 (653749) 653749..653994 + 246 WP_001164966.1 hypothetical protein -
D2J89_RS03225 (654088) 654088..654561 + 474 WP_032287002.1 antirestriction protein -
D2J89_RS03230 (654577) 654577..655053 + 477 WP_001313574.1 RadC family protein -
D2J89_RS03235 (655116) 655116..655337 + 222 WP_000692315.1 DUF987 domain-containing protein -
D2J89_RS03240 (655356) 655356..656000 + 645 WP_000086759.1 hypothetical protein -
D2J89_RS03245 (656050) 656050..656424 + 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
D2J89_RS03250 (656471) 656471..656848 + 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
D2J89_RS03255 (656845) 656845..657337 + 493 Protein_641 DUF5983 family protein -
D2J89_RS03260 (657416) 657416..658404 - 989 Protein_642 IS630 family transposase -
D2J89_RS03265 (658552) 658552..660145 - 1594 Protein_643 IS66 family transposase -
D2J89_RS03270 (660176) 660176..660526 - 351 WP_000624707.1 IS66 family insertion sequence element accessory protein TnpB -
D2J89_RS03275 (660523) 660523..660814 - 292 Protein_645 transposase -
D2J89_RS25745 (660856) 660856..661050 + 195 WP_001313570.1 DUF957 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T110953 WP_000854730.1 NZ_CP032066:656471-656848 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T110953 NZ_CP032066:656471-656848 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT110953 WP_001285481.1 NZ_CP032066:656050-656424 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT110953 NZ_CP032066:656050-656424 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References