Detailed information of TA system TA110930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/Txe-RelB
• Location: 125827..126334
• Replicon: chromosome
• Accession: NZ_CP032049
• Organism: Rickettsia japonica strain LA4/2015

Toxin (Protein)

• Gene name: relB
• Uniprot ID: G4KM08
• Locus tag: D0Z68_RS00785
• Protein ID: WP_014013837.1
• Coordinates: 125827..126090 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: G4KM09
• Locus tag: D0Z68_RS00790
• Protein ID: WP_014013838.1
• Coordinates: 126083..126334 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0Z68_RS00755	120918..121040	+	123	WP_162557097.1	palindromic element RPE4 domain-containing protein	-
D0Z68_RS00760	121328..121714	+	387	Protein_137	hypothetical protein	-
D0Z68_RS00765	121793..122062	+	270	WP_096368100.1	hypothetical protein	-
D0Z68_RS07830	122084..122260	+	177	WP_158309285.1	hypothetical protein	-
D0Z68_RS00770	122364..123758	-	1395	WP_014120498.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
D0Z68_RS00775	123755..124411	-	657	WP_014013835.1	lysophospholipid acyltransferase family protein	-
D0Z68_RS00780	124562..125767	-	1206	WP_014120499.1	pyridoxal phosphate-dependent aminotransferase	-
D0Z68_RS00785	125827..126090	-	264	WP_014013837.1	Txe/YoeB family addiction module toxin	Toxin
D0Z68_RS00790	126083..126334	-	252	WP_014013838.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D0Z68_RS00795	126474..127478	+	1005	WP_014120500.1	methyltransferase domain-containing protein	-
D0Z68_RS00800	127483..128238	+	756	WP_024704441.1	VacJ family lipoprotein	-
D0Z68_RS00805	128258..128857	+	600	WP_014120502.1	phospholipid-binding protein MlaC	-
D0Z68_RS00815	129082..129369	+	288	WP_024704442.1	ribose-phosphate pyrophosphokinase-like domain-containing protein	-
D0Z68_RS07835	129478..129945	+	468	WP_186786268.1	palindromic element RPE1 domain-containing protein	-
D0Z68_RS00830	130090..131157	+	1068	WP_014120503.1	alanine racemase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10590.39 Da
• Isoelectric Point: 9.8255

>T110930 WP_014013837.1 NZ_CP032049:c126090-125827 [Rickettsia japonica]
MIEIEWTLEAAEDLVYWKKYDIKKYERIKLLIKNIQEASFTGIGKPEPLKHILSGLWSRRINHEHRLIYSVNTKQIIIYN
CRFHYKN

Nucleotide

• Length: 264 bp

>T110930 NZ_CP032049:c126090-125827 [Rickettsia japonica]
ATGATTGAGATTGAGTGGACACTAGAAGCAGCGGAGGATTTAGTGTATTGGAAAAAATACGATATTAAAAAATATGAACG
TATTAAACTTCTAATAAAAAATATCCAAGAAGCATCGTTTACAGGTATAGGTAAGCCCGAACCTTTAAAACATATATTAT
CAGGTTTATGGTCACGTAGAATTAACCACGAACATAGACTAATATATTCTGTCAATACTAAACAAATTATAATATATAAT
TGTAGATTTCATTATAAGAATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9537.81 Da
• Isoelectric Point: 9.4884

>AT110930 WP_014013838.1 NZ_CP032049:c126334-126083 [Rickettsia japonica]
MNSISGTSFRKNLSSVLNTVENDHVPYLIKRKNHKNIILLTEEEYESTKETLYLLSNLANANRIKKSIEQAKRGEFAKVN
LDD

Nucleotide

• Length: 252 bp

>AT110930 NZ_CP032049:c126334-126083 [Rickettsia japonica]
ATGAACTCAATTAGCGGCACTTCATTTAGAAAAAACTTAAGCTCTGTACTAAATACCGTAGAAAACGATCATGTCCCTTA
TCTTATTAAAAGAAAGAATCATAAGAATATTATTCTTTTAACCGAAGAAGAATATGAATCTACAAAAGAAACATTATATT
TATTATCTAATCTGGCTAATGCGAATCGAATAAAAAAATCTATCGAACAAGCAAAAAGAGGGGAGTTTGCTAAAGTTAAT
TTAGATGATTGA

Structures

Toxin

• AlphaFold DB: A0A0F3RG12

Antitoxin

• AlphaFold DB: A0A6H3RZ38