Detailed information of TA system TA110927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2325234..2325875
• Replicon: chromosome
• Accession: NZ_CP032011
• Organism: Burkholderia cepacia strain 39628

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C7S14_RS33135
• Protein ID: WP_042973286.1
• Coordinates: 2325537..2325875 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: C7S14_RS33130
• Protein ID: WP_021157290.1
• Coordinates: 2325234..2325533 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7S14_RS33105	2320349..2321620	+	1272	WP_059622368.1	Rieske 2Fe-2S domain-containing protein	-
C7S14_RS33110	2321638..2322123	+	486	WP_048022184.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
C7S14_RS33115	2322144..2322469	+	326	Protein_2057	non-heme iron oxygenase ferredoxin subunit	-
C7S14_RS33120	2322459..2323679	+	1221	WP_192388918.1	FAD-dependent oxidoreductase	-
C7S14_RS33125	2323891..2325177	+	1287	WP_192388920.1	DUF445 domain-containing protein	-
C7S14_RS33130	2325234..2325533	-	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
C7S14_RS33135	2325537..2325875	-	339	WP_042973286.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7S14_RS33140	2326215..2327855	+	1641	WP_021157288.1	acetolactate synthase large subunit	-
C7S14_RS33145	2327884..2329317	+	1434	WP_192388922.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12637.52 Da
• Isoelectric Point: 9.5686

>T110927 WP_042973286.1 NZ_CP032011:c2325875-2325537 [Burkholderia cepacia]
MVDIPQSMPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T110927 NZ_CP032011:c2325875-2325537 [Burkholderia cepacia]
ATGGTTGACATACCGCAATCAATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTT
TGCCGGCCTGCGGGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCGGGCTCGCCGGTCGTCGAAATGCGGATCGACCACGGCCCCGGTTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCTGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGC
GCACCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT110927 WP_021157290.1 NZ_CP032011:c2325533-2325234 [Burkholderia cepacia]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT110927 NZ_CP032011:c2325533-2325234 [Burkholderia cepacia]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCAAAAGCGCACGCATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3