Detailed information of TA system TA110920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5611..6199
• Replicon: plasmid pAhaem11616e
• Accession: NZ_CP032004
• Organism: Acinetobacter haemolyticus strain 11616

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: Ahae11616_RS17215
• Protein ID: WP_060513954.1
• Coordinates: 5611..5898 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: Ahae11616_RS17220
• Protein ID: WP_060513955.1
• Coordinates: 5885..6199 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ahae11616_RS17190	944..1519	+	576	WP_060513917.1	helix-turn-helix domain-containing protein	-
Ahae11616_RS17195	1927..3243	+	1317	WP_060513878.1	AAA family ATPase	-
Ahae11616_RS17200	3258..3929	+	672	WP_060513879.1	hypothetical protein	-
Ahae11616_RS17205	4017..4628	-	612	WP_004740252.1	recombinase family protein	-
Ahae11616_RS17210	4843..5370	+	528	WP_125305375.1	plasmid pRiA4b ORF-3 family protein	-
Ahae11616_RS17215	5611..5898	+	288	WP_060513954.1	BrnT family toxin	Toxin
Ahae11616_RS17220	5885..6199	+	315	WP_060513955.1	BrnA antitoxin family protein	Antitoxin
Ahae11616_RS17225	6426..6743	+	318	WP_004728121.1	hypothetical protein	-
Ahae11616_RS17230	6983..7207	-	225	WP_004758944.1	hypothetical protein	-
Ahae11616_RS17235	7361..7606	+	246	WP_005249014.1	hypothetical protein	-
Ahae11616_RS17240	7892..8653	-	762	WP_057073610.1	hypothetical protein	-
Ahae11616_RS17245	8956..10125	+	1170	WP_125305376.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10694	10694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.63 Da
• Isoelectric Point: 5.2134

>T110920 WP_060513954.1 NZ_CP032004:5611-5898 [Acinetobacter haemolyticus]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISMQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQATKAERDRYEHG

Nucleotide

• Length: 288 bp

>T110920 NZ_CP032004:5611-5898 [Acinetobacter haemolyticus]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACATGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGATGATCCATTACGGATCTCAATGCAGGATAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
GAGTAAAAGGCGTACTAATGCTATTAGTAGCTCACACCATCTTCGATGAAGATGATTGTGAAATCATACGAATCATTAGT
GCAAGACAAGCCACTAAGGCGGAGCGAGATAGATATGAGCACGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12251.85 Da
• Isoelectric Point: 9.4865

>AT110920 WP_060513955.1 NZ_CP032004:5885-6199 [Acinetobacter haemolyticus]
MSTVRYSRKELNEKFSEKQNAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKSQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT110920 NZ_CP032004:5885-6199 [Acinetobacter haemolyticus]
ATGAGCACGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTTAGTGAGAAGCAAAATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ATAATCAGTTCTACCGTCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAATCTCAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTCTGCGAGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA