Detailed information of TA system TA110899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 7531..8119
• Replicon: plasmid pAhaem5227b
• Accession: NZ_CP031989
• Organism: Acinetobacter haemolyticus strain 5227

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: Ahae5227_RS17670
• Protein ID: WP_118901240.1
• Coordinates: 7832..8119 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: Ahae5227_RS17665
• Protein ID: WP_118901243.1
• Coordinates: 7531..7845 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ahae5227_RS17630	3258..3929	+	672	WP_060513879.1	hypothetical protein	-
Ahae5227_RS17635	4017..4628	-	612	WP_004740252.1	recombinase family protein	-
Ahae5227_RS17640	4843..5370	+	528	WP_125305375.1	plasmid pRiA4b ORF-3 family protein	-
Ahae5227_RS17645	5600..5911	+	312	WP_005086328.1	BrnT family toxin	-
Ahae5227_RS17650	5874..6188	+	315	WP_004740255.1	BrnA antitoxin family protein	-
Ahae5227_RS17655	6297..6818	-	522	WP_109292009.1	hypothetical protein	-
Ahae5227_RS17660	6985..7470	+	486	WP_109292001.1	hypothetical protein	-
Ahae5227_RS17665	7531..7845	-	315	WP_118901243.1	BrnA antitoxin family protein	Antitoxin
Ahae5227_RS17670	7832..8119	-	288	WP_118901240.1	BrnT family toxin	Toxin
Ahae5227_RS17675	8348..8665	-	318	WP_005065053.1	hypothetical protein	-
Ahae5227_RS17680	9082..9306	-	225	WP_004676219.1	helix-turn-helix domain-containing protein	-
Ahae5227_RS17685	9456..9701	+	246	WP_004282220.1	hypothetical protein	-
Ahae5227_RS17690	9993..10754	-	762	WP_057073610.1	hypothetical protein	-
Ahae5227_RS17695	11059..12228	+	1170	WP_160124107.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12769	12769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11335.67 Da
• Isoelectric Point: 4.9644

>T110899 WP_118901240.1 NZ_CP031989:c8119-7832 [Acinetobacter haemolyticus]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHD

Nucleotide

• Length: 288 bp

>T110899 NZ_CP031989:c8119-7832 [Acinetobacter haemolyticus]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATGTCTCTTTCGAAACTGCAAG
CCTTGTTTTTGATGATCCATTAAGGATCTCAATCCAAGACAGGCATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACCAAAGCGGAGCGAGATAGATATGAGCATGATTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12284.95 Da
• Isoelectric Point: 8.4842

>AT110899 WP_118901243.1 NZ_CP031989:c7845-7531 [Acinetobacter haemolyticus]
MSMIRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT110899 NZ_CP031989:c7845-7531 [Acinetobacter haemolyticus]
ATGAGCATGATTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTTTGCGGGATGCTATGCTCAAAGAATTAAAAAATCATCAGTAA