Detailed information of TA system TA110887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 6378..6966
• Replicon: plasmid pAhaemAN4c
• Accession: NZ_CP031981
• Organism: Acinetobacter haemolyticus strain AN4

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: AhaeAN4_RS17885
• Protein ID: WP_151800610.1
• Coordinates: 6378..6665 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: AhaeAN4_RS17890
• Protein ID: WP_151800611.1
• Coordinates: 6652..6966 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AhaeAN4_RS17860	1832..2389	+	558	WP_008942535.1	plasmid replication DNA-binding protein	-
AhaeAN4_RS17865	2703..2978	+	276	WP_001133626.1	metal/formaldehyde-sensitive transcriptional repressor	-
AhaeAN4_RS17870	3000..4109	+	1110	WP_000842148.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
AhaeAN4_RS17875	4162..4560	+	399	WP_000235176.1	VOC family protein	-
AhaeAN4_RS17880	5034..5867	+	834	WP_000439570.1	S-formylglutathione hydrolase	-
AhaeAN4_RS17885	6378..6665	+	288	WP_151800610.1	BrnT family toxin	Toxin
AhaeAN4_RS17890	6652..6966	+	315	WP_151800611.1	BrnA antitoxin family protein	Antitoxin
AhaeAN4_RS17895	7193..7510	+	318	WP_004728121.1	hypothetical protein	-
AhaeAN4_RS17900	7750..7974	-	225	WP_004676219.1	helix-turn-helix domain-containing protein	-
AhaeAN4_RS17905	8124..8369	+	246	WP_004282220.1	hypothetical protein	-
AhaeAN4_RS17910	8661..9422	-	762	WP_000425105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10554	10554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.67 Da
• Isoelectric Point: 5.2134

>T110887 WP_151800610.1 NZ_CP031981:6378-6665 [Acinetobacter haemolyticus]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISMQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T110887 NZ_CP031981:6378-6665 [Acinetobacter haemolyticus]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGATGATCCACTGAGGATCTCAATGCAGGATAGACATACCGATGGTGAAGAACGTTGGCAGACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTCACTAAGGCGGAGCGAGATAGATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12242.86 Da
• Isoelectric Point: 8.4842

>AT110887 WP_151800611.1 NZ_CP031981:6652-6966 [Acinetobacter haemolyticus]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVADDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKSQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT110887 NZ_CP031981:6652-6966 [Acinetobacter haemolyticus]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTGCTGACGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ATAATCAGTTCTACCGTCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAATCTCAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTCTGCGAGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA