Detailed information of TA system TA110834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 64008..64678
• Replicon: plasmid unnamed1
• Accession: NZ_CP031935
• Organism: Klebsiella pneumoniae strain GZ-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: D1368_RS27385
• Protein ID: WP_004213072.1
• Coordinates: 64008..64451 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: D1368_RS27390
• Protein ID: WP_004213073.1
• Coordinates: 64448..64678 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1368_RS27350	59419..59694	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
D1368_RS27355	59757..60248	+	492	WP_011251327.1	DM13 domain-containing protein	-
D1368_RS27360	60297..61217	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
D1368_RS27365	61308..61714	+	407	Protein_71	GAF domain-containing protein	-
D1368_RS27370	62229..62864	-	636	Protein_72	response regulator	-
D1368_RS27375	63281..63574	+	294	Protein_73	transposase	-
D1368_RS27380	63578..63994	+	417	Protein_74	restriction endonuclease	-
D1368_RS27385	64008..64451	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1368_RS27390	64448..64678	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1368_RS27395	65286..66419	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
D1368_RS27400	66435..66728	+	294	WP_004213076.1	hypothetical protein	-
D1368_RS27405	66718..66924	-	207	WP_004213077.1	hypothetical protein	-
D1368_RS27410	67276..67566	+	291	WP_004213078.1	hypothetical protein	-
D1368_RS27415	67556..68455	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..198126	198126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T110834 WP_004213072.1 NZ_CP031935:c64451-64008 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T110834 NZ_CP031935:c64451-64008 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT110834 WP_004213073.1 NZ_CP031935:c64678-64448 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT110834 NZ_CP031935:c64678-64448 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620