Detailed information of TA system TA110814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 23511..24154
• Replicon: plasmid unnamed6
• Accession: NZ_CP031923
• Organism: Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: B9S25_RS29745
• Protein ID: WP_024017568.1
• Coordinates: 23738..24154 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: B9S25_RS29740
• Protein ID: WP_001261287.1
• Coordinates: 23511..23741 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9S25_RS29695	19376..20815	+	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
B9S25_RS29700	20882..21025	+	144	Protein_17	transcriptional regulator	-
B9S25_RS29705	21029..21220	+	192	Protein_18	lipid II-degrading bacteriocin	-
B9S25_RS29710	21228..21581	-	354	WP_000864810.1	colicin M immunity protein	-
B9S25_RS29715	21754..22536	-	783	WP_001164205.1	site-specific integrase	-
B9S25_RS29720	22538..22951	-	414	WP_000465041.1	hypothetical protein	-
B9S25_RS29740	23511..23741	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B9S25_RS29745	23738..24154	+	417	WP_024017568.1	type II toxin-antitoxin system VapC family toxin	Toxin
B9S25_RS29750	24316..24861	-	546	WP_001165114.1	hypothetical protein	-
B9S25_RS29755	24929..26085	+	1157	WP_085948186.1	IS3-like element IS600 family transposase	-
B9S25_RS30565	26126..26275	-	150	WP_000955366.1	hypothetical protein	-
B9S25_RS29760	26890..27750	-	861	WP_000704534.1	alpha/beta hydrolase	-
B9S25_RS29765	27878..28264	+	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
B9S25_RS29770	28318..28992	+	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / espP	1..95298	95298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15196.72 Da
• Isoelectric Point: 8.7503

>T110814 WP_024017568.1 NZ_CP031923:23738-24154 [Escherichia coli O26:H11]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDKFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T110814 NZ_CP031923:23738-24154 [Escherichia coli O26:H11]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATAAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT110814 WP_001261287.1 NZ_CP031923:23511-23741 [Escherichia coli O26:H11]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT110814 NZ_CP031923:23511-23741 [Escherichia coli O26:H11]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QFC4