Detailed information of TA system TA110810

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4949039..4949871
• Replicon: chromosome
• Accession: NZ_CP031922
• Organism: Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: B9S25_RS25945
• Protein ID: WP_000854753.1
• Coordinates: 4949039..4949413 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: B9S25_RS25950
• Protein ID: WP_001315620.1
• Coordinates: 4949503..4949871 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9S25_RS25900	4944254..4945360	+	1107	WP_001341302.1	N-acetylneuraminate epimerase	-
B9S25_RS25905	4945425..4946405	+	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
B9S25_RS25910	4946413..4947063	-	651	WP_001037966.1	HNH endonuclease	-
B9S25_RS30415	4948108..4948260	-	153	WP_001280445.1	hypothetical protein	-
B9S25_RS25935	4948345..4948542	-	198	WP_000839281.1	DUF957 domain-containing protein	-
B9S25_RS25940	4948554..4949042	-	489	WP_000777547.1	hypothetical protein	-
B9S25_RS25945	4949039..4949413	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
B9S25_RS25950	4949503..4949871	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B9S25_RS25955	4950034..4950255	-	222	WP_000692345.1	DUF987 domain-containing protein	-
B9S25_RS25960	4950318..4950794	-	477	WP_001186774.1	RadC family protein	-
B9S25_RS25965	4950810..4951295	-	486	WP_000849588.1	antirestriction protein	-
B9S25_RS25970	4951350..4952168	-	819	WP_001234622.1	DUF945 domain-containing protein	-
B9S25_RS25980	4952268..4952501	-	234	WP_001119729.1	DUF905 family protein	-
B9S25_RS25985	4952580..4953035	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T110810 WP_000854753.1 NZ_CP031922:c4949413-4949039 [Escherichia coli O26:H11]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T110810 NZ_CP031922:c4949413-4949039 [Escherichia coli O26:H11]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT110810 WP_001315620.1 NZ_CP031922:c4949871-4949503 [Escherichia coli O26:H11]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT110810 NZ_CP031922:c4949871-4949503 [Escherichia coli O26:H11]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5