Detailed information of TA system TA110808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4349342..4350179
• Replicon: chromosome
• Accession: NZ_CP031922
• Organism: Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: B9S25_RS23075
• Protein ID: WP_000227784.1
• Coordinates: 4349637..4350179 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: B9S25_RS23070
• Protein ID: WP_001297137.1
• Coordinates: 4349342..4349653 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9S25_RS23045	4344362..4345309	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
B9S25_RS23050	4345331..4347322	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
B9S25_RS23055	4347312..4347926	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
B9S25_RS23060	4347926..4348255	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
B9S25_RS23065	4348267..4349157	+	891	WP_000971336.1	heme o synthase	-
B9S25_RS23070	4349342..4349653	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
B9S25_RS23075	4349637..4350179	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
B9S25_RS23080	4350235..4351170	-	936	WP_001375746.1	sel1 repeat family protein	-
B9S25_RS23085	4351578..4352942	+	1365	WP_001000977.1	MFS transporter	-
B9S25_RS23090	4353070..4353561	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
B9S25_RS23095	4353729..4354640	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T110808 WP_000227784.1 NZ_CP031922:4349637-4350179 [Escherichia coli O26:H11]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T110808 NZ_CP031922:4349637-4350179 [Escherichia coli O26:H11]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT110808 WP_001297137.1 NZ_CP031922:4349342-4349653 [Escherichia coli O26:H11]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT110808 NZ_CP031922:4349342-4349653 [Escherichia coli O26:H11]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3