Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4315264..4315882 Replicon chromosome
Accession NZ_CP031922
Organism Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag B9S25_RS22905 Protein ID WP_001291435.1
Coordinates 4315664..4315882 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag B9S25_RS22900 Protein ID WP_000344800.1
Coordinates 4315264..4315638 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9S25_RS22890 4310353..4311546 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
B9S25_RS22895 4311569..4314718 + 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
B9S25_RS22900 4315264..4315638 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
B9S25_RS22905 4315664..4315882 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
B9S25_RS22910 4316054..4316605 + 552 WP_000102564.1 maltose O-acetyltransferase -
B9S25_RS22915 4316721..4317191 + 471 WP_000136192.1 YlaC family protein -
B9S25_RS22920 4317355..4318905 + 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
B9S25_RS22925 4318947..4319300 - 354 WP_000878140.1 DUF1428 domain-containing protein -
B9S25_RS22935 4319678..4319989 + 312 WP_000409911.1 MGMT family protein -
B9S25_RS22940 4320020..4320592 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110807 WP_001291435.1 NZ_CP031922:4315664-4315882 [Escherichia coli O26:H11]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T110807 NZ_CP031922:4315664-4315882 [Escherichia coli O26:H11]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT110807 WP_000344800.1 NZ_CP031922:4315264-4315638 [Escherichia coli O26:H11]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT110807 NZ_CP031922:4315264-4315638 [Escherichia coli O26:H11]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References