Detailed information of TA system TA110787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1385099..1385724
• Replicon: chromosome
• Accession: NZ_CP031922
• Organism: Escherichia coli O26:H11 strain FWSEC0001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B9S25_RS06865
• Protein ID: WP_000911330.1
• Coordinates: 1385326..1385724 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: B9S25_RS06860
• Protein ID: WP_000450524.1
• Coordinates: 1385099..1385326 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9S25_RS06835	1380902..1381372	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
B9S25_RS06840	1381372..1381944	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
B9S25_RS06845	1382090..1382968	+	879	WP_044861983.1	4-hydroxy-tetrahydrodipicolinate synthase	-
B9S25_RS06850	1382985..1384019	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
B9S25_RS06855	1384232..1384945	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
B9S25_RS06860	1385099..1385326	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
B9S25_RS06865	1385326..1385724	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
B9S25_RS06870	1385871..1386734	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
B9S25_RS06875	1386749..1388764	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
B9S25_RS06880	1388838..1389536	+	699	WP_000679812.1	esterase	-
B9S25_RS06885	1389646..1389846	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T110787 WP_000911330.1 NZ_CP031922:1385326-1385724 [Escherichia coli O26:H11]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T110787 NZ_CP031922:1385326-1385724 [Escherichia coli O26:H11]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT110787 WP_000450524.1 NZ_CP031922:1385099-1385326 [Escherichia coli O26:H11]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT110787 NZ_CP031922:1385099-1385326 [Escherichia coli O26:H11]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33