Detailed information of TA system TA110780

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5117725..5118557
• Replicon: chromosome
• Accession: NZ_CP031919
• Organism: Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: CCU01_RS26155
• Protein ID: WP_000854753.1
• Coordinates: 5118183..5118557 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9QGA8
• Locus tag: CCU01_RS26150
• Protein ID: WP_001280948.1
• Coordinates: 5117725..5118093 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU01_RS26120	5113019..5113399	+	381	Protein_4979	DUF932 domain-containing protein	-
CCU01_RS26125	5113909..5115687	+	1779	WP_001243195.1	reverse transcriptase/maturase family protein	-
CCU01_RS26130	5115806..5116246	+	441	Protein_4981	DUF932 domain-containing protein	-
CCU01_RS26135	5116301..5116786	+	486	WP_000849571.1	antirestriction protein	-
CCU01_RS26140	5116802..5117278	+	477	WP_001186726.1	RadC family protein	-
CCU01_RS26145	5117341..5117562	+	222	WP_000692311.1	DUF987 domain-containing protein	-
CCU01_RS26150	5117725..5118093	+	369	WP_001280948.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU01_RS26155	5118183..5118557	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
CCU01_RS26160	5118554..5119042	+	489	WP_000777552.1	hypothetical protein	-
CCU01_RS26165	5119054..5119251	+	198	WP_000839281.1	DUF957 domain-containing protein	-
CCU01_RS26170	5119348..5119908	+	561	WP_044869391.1	DUF4942 domain-containing protein	-
CCU01_RS26175	5119934..5121147	+	1214	WP_136952279.1	IS3 family transposase	-
CCU01_RS26185	5121979..5123517	+	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5092340..5121147	28807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T110780 WP_000854753.1 NZ_CP031919:5118183-5118557 [Escherichia coli O145:NM]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T110780 NZ_CP031919:5118183-5118557 [Escherichia coli O145:NM]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.50 Da
• Isoelectric Point: 7.3991

>AT110780 WP_001280948.1 NZ_CP031919:5117725-5118093 [Escherichia coli O145:NM]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT110780 NZ_CP031919:5117725-5118093 [Escherichia coli O145:NM]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: A0A7U9QGA8