Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4693917..4694467 Replicon chromosome
Accession NZ_CP031919
Organism Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag CCU01_RS24135 Protein ID WP_001160974.1
Coordinates 4693917..4694231 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CCU01_RS24140 Protein ID WP_000125566.1
Coordinates 4694234..4694467 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU01_RS24110 4689592..4690878 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CCU01_RS24115 4690878..4691867 + 990 WP_000241240.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CCU01_RS24120 4691864..4692685 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CCU01_RS24125 4692688..4693065 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CCU01_RS24130 4693072..4693920 + 849 WP_000257203.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CCU01_RS24135 4693917..4694231 - 315 WP_001160974.1 CcdB family protein Toxin
CCU01_RS24140 4694234..4694467 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CCU01_RS24145 4694553..4695032 - 480 WP_024221573.1 type 3 dihydrofolate reductase -
CCU01_RS24150 4695224..4697086 - 1863 WP_000377137.1 glutathione-regulated potassium-efflux system protein KefC -
CCU01_RS24155 4697079..4697609 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CCU01_RS24160 4697720..4699051 - 1332 WP_001183192.1 MFS transporter -
CCU01_RS24165 4699109..4699396 - 288 WP_000203748.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T110778 WP_001160974.1 NZ_CP031919:c4694231-4693917 [Escherichia coli O145:NM]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T110778 NZ_CP031919:c4694231-4693917 [Escherichia coli O145:NM]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT110778 WP_000125566.1 NZ_CP031919:c4694467-4694234 [Escherichia coli O145:NM]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT110778 NZ_CP031919:c4694467-4694234 [Escherichia coli O145:NM]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References