Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1113358..1113941 Replicon chromosome
Accession NZ_CP031919
Organism Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U9LIA0
Locus tag CCU01_RS05605 Protein ID WP_000254747.1
Coordinates 1113606..1113941 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag CCU01_RS05600 Protein ID WP_000581937.1
Coordinates 1113358..1113606 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU01_RS05590 1109697..1110998 + 1302 WP_000046821.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
CCU01_RS05595 1111046..1113280 + 2235 WP_000226815.1 GTP pyrophosphokinase -
CCU01_RS05600 1113358..1113606 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CCU01_RS05605 1113606..1113941 + 336 WP_000254747.1 endoribonuclease MazF Toxin
CCU01_RS05610 1114012..1114803 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
CCU01_RS05615 1115031..1116668 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
CCU01_RS05620 1116756..1118054 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T110763 WP_000254747.1 NZ_CP031919:1113606-1113941 [Escherichia coli O145:NM]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLYVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T110763 NZ_CP031919:1113606-1113941 [Escherichia coli O145:NM]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTATGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT110763 WP_000581937.1 NZ_CP031919:1113358-1113606 [Escherichia coli O145:NM]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT110763 NZ_CP031919:1113358-1113606 [Escherichia coli O145:NM]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LIA0

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References