Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 984769..985423 Replicon chromosome
Accession NZ_CP031919
Organism Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CCU01_RS05065 Protein ID WP_000244777.1
Coordinates 985016..985423 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CCU01_RS05060 Protein ID WP_000354046.1
Coordinates 984769..985035 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU01_RS05040 980857..982290 - 1434 WP_024221758.1 6-phospho-beta-glucosidase BglA -
CCU01_RS05045 982335..982646 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CCU01_RS05050 982810..983469 + 660 WP_000250294.1 hemolysin III family protein -
CCU01_RS05055 983546..984526 - 981 WP_000886093.1 tRNA-modifying protein YgfZ -
CCU01_RS05060 984769..985035 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CCU01_RS05065 985016..985423 + 408 WP_000244777.1 toxin CptA Toxin
CCU01_RS05070 985463..985984 - 522 WP_001055874.1 flavodoxin FldB -
CCU01_RS05075 986096..986992 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CCU01_RS05080 987017..987727 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CCU01_RS05085 987733..989466 + 1734 WP_000813202.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T110762 WP_000244777.1 NZ_CP031919:985016-985423 [Escherichia coli O145:NM]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T110762 NZ_CP031919:985016-985423 [Escherichia coli O145:NM]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT110762 WP_000354046.1 NZ_CP031919:984769-985035 [Escherichia coli O145:NM]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT110762 NZ_CP031919:984769-985035 [Escherichia coli O145:NM]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References