Detailed information of TA system TA110760

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 774976..775813
• Replicon: chromosome
• Accession: NZ_CP031919
• Organism: Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: CCU01_RS03965
• Protein ID: WP_000854687.1
• Coordinates: 775433..775813 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LJJ3
• Locus tag: CCU01_RS03960
• Protein ID: WP_001443392.1
• Coordinates: 774976..775344 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU01_RS03925	770082..770465	+	384	Protein_755	DUF932 domain-containing protein	-
CCU01_RS03930	770975..772753	+	1779	WP_001243195.1	reverse transcriptase/maturase family protein	-
CCU01_RS03935	772872..773312	+	441	Protein_757	DUF932 domain-containing protein	-
CCU01_RS03940	773312..773557	+	246	WP_000680587.1	hypothetical protein	-
CCU01_RS03945	773651..774124	+	474	WP_001444656.1	antirestriction protein	-
CCU01_RS03950	774140..774616	+	477	WP_001186728.1	RadC family protein	-
CCU01_RS03955	774679..774900	+	222	WP_000692312.1	DUF987 domain-containing protein	-
CCU01_RS03960	774976..775344	+	369	WP_001443392.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU01_RS03965	775433..775813	+	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
CCU01_RS03970	775810..776298	+	489	WP_001054228.1	hypothetical protein	-
CCU01_RS03975	776318..776515	+	198	WP_000772026.1	DUF957 domain-containing protein	-
CCU01_RS03980	776600..777445	+	846	WP_001274557.1	DUF4942 domain-containing protein	-
CCU01_RS03990	777745..778251	+	507	WP_000228947.1	G/U mismatch-specific DNA glycosylase	-
CCU01_RS03995	778330..780171	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T110760 WP_000854687.1 NZ_CP031919:775433-775813 [Escherichia coli O145:NM]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T110760 NZ_CP031919:775433-775813 [Escherichia coli O145:NM]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.54 Da
• Isoelectric Point: 6.2049

>AT110760 WP_001443392.1 NZ_CP031919:774976-775344 [Escherichia coli O145:NM]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT110760 NZ_CP031919:774976-775344 [Escherichia coli O145:NM]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTAGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67

Antitoxin

• AlphaFold DB: A0A7U9LJJ3