Detailed information of TA system TA110757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 346652..347452
• Replicon: chromosome
• Accession: NZ_CP031919
• Organism: Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: U9Y257
• Locus tag: CCU01_RS01685
• Protein ID: WP_000342447.1
• Coordinates: 346925..347452 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: U9Y285
• Locus tag: CCU01_RS01680
• Protein ID: WP_001277109.1
• Coordinates: 346652..346918 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU01_RS01660	342310..342978	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
CCU01_RS01665	342971..344029	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
CCU01_RS01670	344274..345128	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
CCU01_RS01675	345399..346502	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
CCU01_RS01680	346652..346918	+	267	WP_001277109.1	DUF1778 domain-containing protein	Antitoxin
CCU01_RS01685	346925..347452	+	528	WP_000342447.1	GNAT family N-acetyltransferase	Toxin
CCU01_RS01690	347449..347832	-	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
CCU01_RS01695	348257..349366	+	1110	WP_001400431.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
CCU01_RS01700	349414..350340	+	927	WP_000003013.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
CCU01_RS01705	350337..351614	+	1278	WP_000803782.1	branched chain amino acid ABC transporter permease LivM	-
CCU01_RS01710	351611..352378	+	768	WP_000082096.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.63 Da
• Isoelectric Point: 7.0284

>T110757 WP_000342447.1 NZ_CP031919:346925-347452 [Escherichia coli O145:NM]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T110757 NZ_CP031919:346925-347452 [Escherichia coli O145:NM]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCAGTAGGTATTCACGGTCTTTTTGTAG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCGTTGGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9938.37 Da
• Isoelectric Point: 10.0863

>AT110757 WP_001277109.1 NZ_CP031919:346652-346918 [Escherichia coli O145:NM]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Nucleotide

• Length: 267 bp

>AT110757 NZ_CP031919:346652-346918 [Escherichia coli O145:NM]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LUM2

Antitoxin

• AlphaFold DB: A0A7U9LUN0