Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 303838..304469 Replicon chromosome
Accession NZ_CP031919
Organism Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag CCU01_RS01430 Protein ID WP_001259387.1
Coordinates 303838..304113 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CCU01_RS01435 Protein ID WP_000593555.1
Coordinates 304110..304469 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU01_RS01415 298842..299909 + 1068 WP_000361517.1 HlyD family secretion protein -
CCU01_RS01420 299906..302641 + 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
CCU01_RS01425 302641..303765 + 1125 WP_001314210.1 ABC transporter permease -
CCU01_RS01430 303838..304113 + 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
CCU01_RS01435 304110..304469 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CCU01_RS01440 304519..305379 - 861 WP_001131788.1 class II aldolase -
CCU01_RS01445 305411..305680 - 270 WP_000933061.1 HPr family phosphocarrier protein -
CCU01_RS01450 305670..307178 - 1509 WP_001400448.1 carbohydrate kinase -
CCU01_RS01455 307171..308529 - 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
CCU01_RS01460 308606..308887 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
CCU01_RS01465 308884..309357 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T110756 WP_001259387.1 NZ_CP031919:303838-304113 [Escherichia coli O145:NM]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T110756 NZ_CP031919:303838-304113 [Escherichia coli O145:NM]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT110756 WP_000593555.1 NZ_CP031919:304110-304469 [Escherichia coli O145:NM]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT110756 NZ_CP031919:304110-304469 [Escherichia coli O145:NM]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References