Detailed information of TA system TA110755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 70688..71289
• Replicon: plasmid unnamed11
• Accession: NZ_CP031917
• Organism: Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)

• Gene name: doc
• Uniprot ID: I2USK8
• Locus tag: CCU02_RS28845
• Protein ID: WP_001216042.1
• Coordinates: 70688..71068 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: I2USI4
• Locus tag: CCU02_RS28850
• Protein ID: WP_001190710.1
• Coordinates: 71068..71289 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU02_RS28815	66817..67125	+	309	WP_000349257.1	hypothetical protein	-
CCU02_RS28825	67548..68085	+	538	Protein_62	dUTP diphosphatase	-
CCU02_RS28830	68278..68754	+	477	WP_169301912.1	DUF2829 domain-containing protein	-
CCU02_RS28835	68761..69567	+	807	WP_044868339.1	hypothetical protein	-
CCU02_RS28840	69597..70652	+	1056	WP_136868083.1	hypothetical protein	-
CCU02_RS28845	70688..71068	-	381	WP_001216042.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CCU02_RS28850	71068..71289	-	222	WP_001190710.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CCU02_RS28855	71362..71751	-	390	WP_000506730.1	DNA repair protein	-
CCU02_RS28860	71982..72521	-	540	WP_136868084.1	toprim domain-containing protein	-
CCU02_RS28865	72518..72823	-	306	WP_000988220.1	hypothetical protein	-
CCU02_RS28875	73091..73840	-	750	WP_044868336.1	hypothetical protein	-
CCU02_RS28880	73837..74046	-	210	WP_044868334.1	hypothetical protein	-
CCU02_RS28885	74071..74364	-	294	WP_000267996.1	hypothetical protein	-
CCU02_RS28895	74351..75503	-	1153	Protein_74	ASCH domain-containing protein	-
CCU02_RS28905	75835..76013	-	179	Protein_75	hypothetical protein	-
CCU02_RS28910	76015..76201	-	187	Protein_76	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95228	95228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.6406

>T110755 WP_001216042.1 NZ_CP031917:c71068-70688 [Escherichia coli O45:H2]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI

Nucleotide

• Length: 381 bp

>T110755 NZ_CP031917:c71068-70688 [Escherichia coli O45:H2]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8147.13 Da
• Isoelectric Point: 4.7708

>AT110755 WP_001190710.1 NZ_CP031917:c71289-71068 [Escherichia coli O45:H2]
MQSINFRTARGNLSELLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT110755 NZ_CP031917:c71289-71068 [Escherichia coli O45:H2]
ATGCAGTCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAATTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAACCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAGGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: I2USK8

Antitoxin

• AlphaFold DB: A0A348ERB2