Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5246005..5246636 Replicon chromosome
Accession NZ_CP031916
Organism Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag CCU02_RS27090 Protein ID WP_001259385.1
Coordinates 5246361..5246636 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag CCU02_RS27085 Protein ID WP_000593554.1
Coordinates 5246005..5246364 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU02_RS27055 5241117..5241590 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
CCU02_RS27060 5241587..5241868 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
CCU02_RS27065 5241945..5243303 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
CCU02_RS27070 5243296..5244804 + 1509 WP_001298718.1 carbohydrate kinase -
CCU02_RS27075 5244794..5245063 + 270 WP_000933060.1 HPr family phosphocarrier protein -
CCU02_RS27080 5245095..5245955 + 861 WP_001131783.1 class II aldolase -
CCU02_RS27085 5246005..5246364 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CCU02_RS27090 5246361..5246636 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
CCU02_RS27095 5246709..5247833 - 1125 WP_001216257.1 ABC transporter permease -
CCU02_RS27100 5247833..5250568 - 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
CCU02_RS27105 5250565..5251632 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T110753 WP_001259385.1 NZ_CP031916:c5246636-5246361 [Escherichia coli O45:H2]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T110753 NZ_CP031916:c5246636-5246361 [Escherichia coli O45:H2]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT110753 WP_000593554.1 NZ_CP031916:c5246364-5246005 [Escherichia coli O45:H2]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT110753 NZ_CP031916:c5246364-5246005 [Escherichia coli O45:H2]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References