Detailed information of TA system TA110751

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4777022..4777859
• Replicon: chromosome
• Accession: NZ_CP031916
• Organism: Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: CCU02_RS24630
• Protein ID: WP_000854687.1
• Coordinates: 4777022..4777402 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LJJ3
• Locus tag: CCU02_RS24635
• Protein ID: WP_001443392.1
• Coordinates: 4777491..4777859 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU02_RS24600	4772664..4774505	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
CCU02_RS24605	4774584..4775090	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
CCU02_RS24615	4775390..4776235	-	846	WP_001274557.1	DUF4942 domain-containing protein	-
CCU02_RS24620	4776320..4776517	-	198	WP_000772026.1	DUF957 domain-containing protein	-
CCU02_RS24625	4776537..4777025	-	489	WP_001054229.1	hypothetical protein	-
CCU02_RS24630	4777022..4777402	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
CCU02_RS24635	4777491..4777859	-	369	WP_001443392.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU02_RS24640	4777935..4778156	-	222	WP_000692312.1	DUF987 domain-containing protein	-
CCU02_RS24645	4778219..4778695	-	477	WP_001186717.1	RadC family protein	-
CCU02_RS24650	4778711..4779184	-	474	WP_001368734.1	antirestriction protein	-
CCU02_RS24655	4779278..4779523	-	246	WP_001164974.1	hypothetical protein	-
CCU02_RS24660	4779523..4779963	-	441	Protein_4697	DUF932 domain-containing protein	-
CCU02_RS24665	4780082..4781860	-	1779	WP_001243195.1	reverse transcriptase/maturase family protein	-
CCU02_RS24670	4782370..4782753	-	384	Protein_4699	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T110751 WP_000854687.1 NZ_CP031916:c4777402-4777022 [Escherichia coli O45:H2]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T110751 NZ_CP031916:c4777402-4777022 [Escherichia coli O45:H2]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.54 Da
• Isoelectric Point: 6.2049

>AT110751 WP_001443392.1 NZ_CP031916:c4777859-4777491 [Escherichia coli O45:H2]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT110751 NZ_CP031916:c4777859-4777491 [Escherichia coli O45:H2]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTAGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67

Antitoxin

• AlphaFold DB: A0A7U9LJJ3