Detailed information of TA system TA110750

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4626449..4627247
• Replicon: chromosome
• Accession: NZ_CP031916
• Organism: Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0F3UPG9
• Locus tag: CCU02_RS23840
• Protein ID: WP_000854698.1
• Coordinates: 4626870..4627247 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0F3T7G0
• Locus tag: CCU02_RS23835
• Protein ID: WP_000017094.1
• Coordinates: 4626449..4626823 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU02_RS23795	4621477..4621635	+	159	WP_001369071.1	DUF905 family protein	-
CCU02_RS23805	4621790..4622170	+	381	Protein_4528	DUF932 domain-containing protein	-
CCU02_RS23810	4622680..4624458	+	1779	WP_001243195.1	reverse transcriptase/maturase family protein	-
CCU02_RS23815	4624577..4625017	+	441	Protein_4530	DUF932 domain-containing protein	-
CCU02_RS23820	4625108..4625593	+	486	WP_000213700.1	antirestriction protein	-
CCU02_RS23825	4625608..4626084	+	477	WP_001186195.1	RadC family protein	-
CCU02_RS23830	4626153..4626374	+	222	WP_001220316.1	DUF987 domain-containing protein	-
CCU02_RS23835	4626449..4626823	+	375	WP_000017094.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU02_RS23840	4626870..4627247	+	378	WP_000854698.1	TA system toxin CbtA family protein	Toxin
CCU02_RS23845	4627244..4627732	+	489	WP_001054226.1	hypothetical protein	-
CCU02_RS23850	4627744..4627941	+	198	WP_000839251.1	DUF957 domain-containing protein	-
CCU02_RS23855	4628026..4628868	+	843	WP_001280439.1	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13975.94 Da
• Isoelectric Point: 7.9087

>T110750 WP_000854698.1 NZ_CP031916:4626870-4627247 [Escherichia coli O45:H2]
MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T110750 NZ_CP031916:4626870-4627247 [Escherichia coli O45:H2]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13732.42 Da
• Isoelectric Point: 5.4488

>AT110750 WP_000017094.1 NZ_CP031916:4626449-4626823 [Escherichia coli O45:H2]
VSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT110750 NZ_CP031916:4626449-4626823 [Escherichia coli O45:H2]
GTGTCAGGCACACTCCATGAGACAAATTATCCCAACGACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAATTACATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0F3UPG9

Antitoxin

• AlphaFold DB: A0A0F3T7G0