Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4507962..4508616 Replicon chromosome
Accession NZ_CP031916
Organism Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag CCU02_RS23220 Protein ID WP_000244772.1
Coordinates 4507962..4508369 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CCU02_RS23225 Protein ID WP_000354046.1
Coordinates 4508350..4508616 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU02_RS23200 4503919..4505652 - 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -
CCU02_RS23205 4505658..4506368 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CCU02_RS23210 4506393..4507289 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CCU02_RS23215 4507401..4507922 + 522 WP_001055874.1 flavodoxin FldB -
CCU02_RS23220 4507962..4508369 - 408 WP_000244772.1 protein YgfX Toxin
CCU02_RS23225 4508350..4508616 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CCU02_RS23230 4508859..4509839 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CCU02_RS23240 4510092..4511072 + 981 WP_000399680.1 IS110-like element IS621 family transposase -
CCU02_RS23245 4511195..4511854 - 660 WP_000250274.1 hemolysin III family protein -
CCU02_RS23250 4512018..4512329 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4510092..4511072 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T110749 WP_000244772.1 NZ_CP031916:c4508369-4507962 [Escherichia coli O45:H2]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T110749 NZ_CP031916:c4508369-4507962 [Escherichia coli O45:H2]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT110749 WP_000354046.1 NZ_CP031916:c4508616-4508350 [Escherichia coli O45:H2]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT110749 NZ_CP031916:c4508616-4508350 [Escherichia coli O45:H2]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References