Detailed information of TA system TA110747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3995881..3996506
• Replicon: chromosome
• Accession: NZ_CP031916
• Organism: Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCU02_RS20665
• Protein ID: WP_000911330.1
• Coordinates: 3995881..3996279 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CCU02_RS20670
• Protein ID: WP_000450524.1
• Coordinates: 3996279..3996506 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU02_RS20645	3991759..3991959	+	201	WP_000383836.1	YpfN family protein	-
CCU02_RS20650	3992069..3992767	-	699	WP_000679812.1	esterase	-
CCU02_RS20655	3992841..3994856	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
CCU02_RS20660	3994871..3995734	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
CCU02_RS20665	3995881..3996279	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CCU02_RS20670	3996279..3996506	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CCU02_RS20675	3996660..3997373	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CCU02_RS20680	3997586..3998620	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CCU02_RS20685	3998637..3999515	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CCU02_RS20690	3999661..4000233	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CCU02_RS20695	4000233..4000703	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T110747 WP_000911330.1 NZ_CP031916:c3996279-3995881 [Escherichia coli O45:H2]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T110747 NZ_CP031916:c3996279-3995881 [Escherichia coli O45:H2]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT110747 WP_000450524.1 NZ_CP031916:c3996506-3996279 [Escherichia coli O45:H2]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT110747 NZ_CP031916:c3996506-3996279 [Escherichia coli O45:H2]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33