Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1355157..1355775 Replicon chromosome
Accession NZ_CP031916
Organism Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CCU02_RS06525 Protein ID WP_001291435.1
Coordinates 1355157..1355375 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CCU02_RS06530 Protein ID WP_000344800.1
Coordinates 1355401..1355775 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU02_RS06490 1350446..1351018 + 573 WP_000779826.1 lipoprotein -
CCU02_RS06495 1351049..1351360 - 312 WP_000409911.1 MGMT family protein -
CCU02_RS06505 1351739..1352092 + 354 WP_000878141.1 DUF1428 family protein -
CCU02_RS06510 1352134..1353684 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CCU02_RS06515 1353848..1354318 - 471 WP_000136192.1 YlaC family protein -
CCU02_RS06520 1354434..1354985 - 552 WP_000102564.1 maltose O-acetyltransferase -
CCU02_RS06525 1355157..1355375 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CCU02_RS06530 1355401..1355775 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CCU02_RS06535 1356321..1359470 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CCU02_RS06540 1359493..1360686 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110729 WP_001291435.1 NZ_CP031916:c1355375-1355157 [Escherichia coli O45:H2]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T110729 NZ_CP031916:c1355375-1355157 [Escherichia coli O45:H2]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT110729 WP_000344800.1 NZ_CP031916:c1355775-1355401 [Escherichia coli O45:H2]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT110729 NZ_CP031916:c1355775-1355401 [Escherichia coli O45:H2]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References