Detailed information of TA system TA110726

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 704382..705157
• Replicon: chromosome
• Accession: NZ_CP031916
• Organism: Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: CCU02_RS03350
• Protein ID: WP_001193489.1
• Coordinates: 704786..705157 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7V7JFU6
• Locus tag: CCU02_RS03345
• Protein ID: WP_001059301.1
• Coordinates: 704382..704747 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU02_RS03310	700419..701135	+	717	WP_000174910.1	WYL domain-containing protein	-
CCU02_RS03315	701171..701623	+	453	WP_000734322.1	hypothetical protein	-
CCU02_RS03320	701695..702168	+	474	WP_001298943.1	hypothetical protein	-
CCU02_RS03325	702288..703109	+	822	WP_001234408.1	DUF945 domain-containing protein	-
CCU02_RS03330	703140..703583	+	444	WP_001096016.1	antirestriction protein	-
CCU02_RS03335	703596..704138	+	543	WP_015740440.1	DNA repair protein RadC	-
CCU02_RS03340	704135..704356	+	222	WP_000691983.1	DUF987 domain-containing protein	-
CCU02_RS03345	704382..704747	+	366	WP_001059301.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU02_RS03350	704786..705157	+	372	WP_001193489.1	TA system toxin CbtA family protein	Toxin
CCU02_RS03370	706312..707292	-	981	WP_000991444.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
CCU02_RS03375	707357..708463	-	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
CCU02_RS03380	708483..709199	-	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	698331..714726	16395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13875.97 Da
• Isoelectric Point: 7.6717

>T110726 WP_001193489.1 NZ_CP031916:704786-705157 [Escherichia coli O45:H2]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYERVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Nucleotide

• Length: 372 bp

>T110726 NZ_CP031916:704786-705157 [Escherichia coli O45:H2]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACGGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.9530

>AT110726 WP_001059301.1 NZ_CP031916:704382-704747 [Escherichia coli O45:H2]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT110726 NZ_CP031916:704382-704747 [Escherichia coli O45:H2]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V7JFU6